Double Your Donation and Make a Difference For Marine Conservation

Photo by Jonathan Lavan.

Earlier this month, for World Oceans Day, the Curtis and Edith Munson Foundation celebrated by pledging to match contributions to REEF dollar for dollar, up to $30,000! Our campaign to raise funds for protecting Nassau Grouper, controlling invasive Lionfish, and inspiring citizen science through the Volunteer Fish Survey Project is off to a great start. But we still need your help to reach our goal in the next 30 days. If you haven't yet had a chance, please contribute today. You can double your donation in the upcoming month by contributing online through our secure web form. Or you can print the donation form and mail or fax your donation, or call our staff at REEF headquarters (305-852-0030).

Contributions from members like you fuel the success of our programs. With your donation, we can expand our new online "Fishinars," which are growing rapidly in popularity. We can continue to fund lionfish education and outreach efforts, such as the Lionfish Cookbook, training and handling workshops, and derbies. Our staff can also keep working with Cayman Islands officials after the recent victory that extended the ban on fishing in Nassau Grouper spawning aggregations. These are just some highlights of REEF accomplishments that are funded by individual contributions. With a chance to double your donation, no gift is too small!

Putting It To Work: New Publications on Lionfish

Photo by Carol Cox.

REEF Director of Special Projects, Lad Akins, has co-authored several recent scientific publications on the invasive lionfish in the western Atlantic, including:

-Diet richness of invasive Indo-Pacific lionfish revealed by DNA barcoding. Marine Ecology Progress Series. Significant research by REEF researchers and others has been conducted looking at stomach contents of lionfish to identify prey. However, relatively few prey species have been identified because of the challenge of identifying partly digested prey. The authors of this study addressed this issue by DNA-barcoding unidentifiable fish items from the stomachs of 130 lionfish. They identified 37 prey species, half of which had previously not been recorded as lionfish prey.

-Rapid invasion of Indo-Pacific lionfishes (Pterois volitans and Pterois miles) in the Florida Keys, USA: evidence from multiple pre- and post-invasion data sets. Bulletin of Marine Science. This paper uses data from the 20,000+ REEF surveys conducted in Florida since the early 1990s, along with other long-term data sources, to document the appearance and rapid spread of lionfishes in the Florida Keys. Between 2009 and 2011, lionfish frequency of occurrence, abundance, and biomass increased rapidly, increasing three- to six-fold between 2010 and 2011 alone.

- Habitat complexity and fish size affect the detection of Indo-Pacific lionfish on invaded coral reefs. Coral Reefs. This paper explores detectability rates of lionfish using underwater visual census methods such as belt transects and stationary visual census. Knowing the error in these methods specficially for lionfish is necessary to help study this invasive species in the western Atlantic. The authors found that the two census methods detect fewer than 30% of lionfish present in an area and, in more than 50% of the cases, fail to detect any lionfish when one or more indivudals are actually present.

For a complete list of publications featuring REEF data, visit

REEF Joins in Submersible Expedition to Assess the Lionfish Invasion Beyond Diving Depths

REEF Affiliate Scientist, Dr. Stephanie Green inside the Antipodes sub.
Several dozen invasive lionfish call an artificial reef in South florida, the Bill Boyd, home off.

REEF's Director of Special Projects, Lad Akins, and REEF affiliate scientist Dr. Stephanie Green (Oregon State University) and REEF Advisory Panel member Dr. Steve Gittings (NOAA National Marine Sanctuaries) participated in the first submersible expedition to assess the lionfish invasion on deep marine habitats off South Florida June 27-29. While REEF and other scientists have studied lionfish in shallow habitats, the Antipodes lionfish expedition gave scientists the opportunity to learn about lionfish populations far below recreational diving limits. The five person submersible is capable of descending to 300 m (1,000 ft) deep and has large acrylic domes that allow for observations and photography.

During the expedition, team members including Dr. Gittings and Dr. Green completed dives to 300ft in the submersible to look for lionfish on both natural rocky and artificial reefs, including the 209ft-long cargo ship Bill Boyd. Both scientists sighted dozens of invasive lionfish in all habitat types during the dive, highlighted by view of the stern of the wreck holding dozens of lionfish. Dr. Green also conducted a number of REEF surveys to document the native fish community in areas invaded by lionfish, sighting a number of reef fishes that are often only found below recreational dive limits, including snowy grouper, roughtongue bass, red barbier, short bigeye, and bank butterflyfish.

The project, hosted by NOVA Southeastern University, was led by OceanGate Inc. and included participants from Florida Fish and Wildlife Conservation Commission, University of Miami, NOVA, and Guy Harvey Foundation, and others. On Saturday following the expedition dives, Lad Akins, Dr. Green, and Dr. Gittings met with media and the public in a half-day summit to discuss the invasion and potential actions to manage lionfish populations in areas that can't regularly be accessed by divers. The summit concluded with a lionfish filleting demonstration by Lad, and a tasting of lionfish ceviche prepared by Kareem Anguin, Executive Chef, The Oceanaire Seafood Room. See the expedition website for more information.

As part of a Florida Sea Grant funded project, REEF is working this summer to assess deepwater lionfish populations in the Florida Keys using ROVs and technical divers.

Please Support REEF and Our Important Marine Conservation Work

Be a part of our new Giving Reef! Donate $500 or more during our winter fundraising campaign.

We want to extend a special thanks to our members who have already made a donation during our Winter Fundraising Campaign. If you haven't yet, please take a moment to support REEF's critical marine conservation work. You can contribute securely online at or call REEF Headquarters at 305-852-0030.

With your support, we will build on twenty years of success. In 2014, REEF plans to: 

  • Encourage use of REEF data to provide species and habitat protections, like those afforded this year to Giant Pacific Octopus in Washington State, Hogfish, Goliath Grouper, and Yellowtail Snapper populations
  • Promote the new fish and invertebrate monitoring program in the South Atlantic States
  • Expand the Volunteer Fish Survey Project to Australia, the Coral Triangle, the North East Atlantic, and the Mediterranean
  • Continue the Nassau Grouper educational program and analyze data collected this year from recently deployed underwater microphones
  • Lead the charge in addressing the lionfish invasion in the Caribbean and Atlantic

Give a gift to our oceans by supporting REEF programs. This year, we also have gifts to give in appreciation of your donation, which include a print of a limited edition, signed print of Sailfin Blenny ($250 or more), acknowledgement on the Giving REEF ($500 or more), and a special webinar with Ned and Anna DeLoach ($1,000 or more).

Putting It To Work: New Publication on Nassau Grouper Populations in the Caribbean

A Nassau Grouper at the spawning aggregation on Little Cayman, which is the focus of research in REEF's Grouper Moon Project. Photo by Christy Pattengill-Semmens.

REEF Grouper Moon scientists co-authored a recent groundbreaking paper in the journal PLoS One that highlights the importance of regional conservation efforts aimed at spawning aggregations in the Caribbean. This study evaluated genetic connectedness between Nassau Grouper populations throughout the Caribbean using DNA markers. The authors obtained genetic tissue samples from 620 Nassau Grouper from 19 sites across 9 countries, including the Cayman Islands. They found evidence for strong genetic differentiation among Nassau Grouper subpopulations throughout the Caribbean. These results suggest that, despite a lack of physical barriers, Nassau Grouper form multiple distinct sub-populations in the Caribbean Sea. Oceanography (regional currents, eddies) likely plays an important role in retaining larvae close to spawning sites at both local and regional spatial scales. These findings highlight the importance of conservation initiatives such at REEF's Grouper Moon program in the Cayman Islands. A PDF of the paper is available online here. You can see a complete list of all scientific papers that have included data from REEF programs at

The full citation of the paper is: Jackson AM, Semmens BX, Sadovy de Mitcheson Y, Nemeth RS, Heppell SA, et al. (2014) Population Structure and Phylogeography in Nassau Grouper (Epinephelus striatus), a Mass-Aggregating Marine Fish. PLoS ONE 9(5): e97508. doi:10.1371/journal.pone.0097508

The Faces of REEF: Nick Brilliande

A Whitetip Reef Shark - one of Nick's memorable finds on a recent survey. Photo by Jim Spears.

REEF members are at the heart of our grassroots marine conservation programs. Over 50,000 divers, snorkelers, students, and armchair naturalists stand behind our mission.

This month we highlight Nick Brilliande. He has been a REEF member since 2011. An active surveyor who lives on Oahu, Hawaii, Nick has conducted 50 surveys to date and is a member of REEF's Advanced Assessment Team for the Hawaii region. Here's what he had to say about REEF:

How did you become involved with REEF?

The first time I heard about REEF was through a group called Reef Watch Waikiki. I attended some talks by REEF members Cassidy Lum and Jennifer Barrett describing what REEF does and how to survey fish. I answered a few questions and made some comments on fish, which impressed both Cassidy and Jen. Then came the time to try it out and I did. I had fun doing it, but it was also an excuse to look at fish, which I always find fascinating. After that, I became a member, went out to survey when I could, and slowly made my way up to an Expert Level 4/5 surveyor.

What inspires you to complete REEF surveys? What is the most interesting thing you learned doing a REEF fish survey?

I am always curious as to how the environment changes over time and how those changes affects the species that live there. The ocean is always different every day in some way or another; you never have the same type of conditions or species.

Do you have any surveying, fishwatching, or identification tips for REEF members?

When doing a fish survey, having an extra pair of eyes does help, but you want to be patient. The fish initially view you as a threat, but wait a little and eventually they will get used to you enough to come out and be able to see them. Let the animals make the first moves.

When learning fish for the first time, do not jump around families. The only thing that will accomplish is a huge headache. Take one family, learn the different species of fish one at a time, then quiz yourself to see if you actually know one species from another. Rinse and repeat. As long as you are out and about, you will never forget a fish's face. As mentioned, patience is key. Let them come out on their terms and let them make the first moves. One thing that seems to work for me is keeping my hands and arms to my side while snorkeling or diving - fish seem to view this as less threatening than flailing arms back and forth or having arms wide out.

What is your most memorable fish find and why? Is there a fish you would really like to see?

There are a few finds I remember. One was in Pokai Bay on O'ahu. Here, I witnessed a female Whitley's Boxfish picking at a turtle with a large tumor beside his mouth. This fish was picking at the tumor, but I still have no idea as to the purpose of this. At this same location on the same day I found my first lobster molt, a Slipper Lobster molt. Another encounter I still remember is in Kealakekua Bay on the Big Island of Hawaii. There were three notable encounters on the same day: two Longnose Butterflyfish, one of which was in a rare dark coloration alongside the other, which was in it's typical yellow coloration, a partial albino Yellow Tang in very shallow water, and a very sleepy Whitetip Reef Shark, which I was able to get very close to without disturbing him.

As far as animals I would like to see, that list would be almost half a page long. A few notable ones would include a Whale Shark, a Dragon Moray Eel, a Hawaiian Monk Seal underwater (I've seen them numerous times on beaches or them swimming around viewed from a boat or shore), and a Hawksbill Sea Turtle.

One Week Left in Summer Matching Campaign

REEF’s Summer Donation Matching Campaign is winding down, but we still need your support to reach our goal! Please consider making your donation today - click here to donate online! We are $12,000 from our goal of $60,000, and we know we can count on the support of our members. Thanks to a generous matching pledge from three of our supporting foundations, your donation will be doubled. Your support helps ensure that we can continue the critical work to protect our world oceans through education and research. Please consider donating today to help us reach our fundraising goal. Every donation, no matter how small, makes double the difference!

A highlight of the summer for us has been our new REEF's Ocean Explorers Camp. From studying mangroves to completing mock health surveys on sea turtles and investigating ocean creatures, these young REEF Explorers had a blast! Just like you, we are committed to educating the public so marine conservation continues well into the future. Education is a component that runs through all of REEF's programs and is essential to ensuring the success of our core citizen science projects. Don't forget that every dollar given is matched by our generous supporters this summer. Please make a today donation at!

And a big fishy thank you to all of our members who have already donated this summer.

Lionfish - What We Know and What We're Learning

Lionfish photo by Tom DeMayo
Juvenile Lionfish photo by Tom DeMayo

If you’ve read recent REEF releases, you’ve heard the news that Indo-pacific lionfish are now well established along the eastern US coast and throughout the Bahamas. REEF has been and continues to work with researchers to learn as much as we can in order to most effectively address the invasion. Since January of this year, REEF has organized and led 5 week-long projects in the Bahamas to document the extent of the invasion and gather samples and information needed by NOAA and Bahamian researchers.

Here is what we’ve found:

  • Lionfish are being found as deep as 350’ and as shallow as 2’.
  • Lionfish have been documented in almost all habitat types including patch reefs, artificial reefs, walls, and even mangroves
  • Lionfish have been captured as small as 25mm and as large as 389mm
  • Most lionfish have been in the 200mm size range
  • Lionfish prey has included fish, shrimp and crabs
  • Lionfish appear to have high site fidelity (they don’t move much)
  • Lionfish appear to be reproducing year-round in Bahamian waters
  • The lionfish invasion appears to have come from a small founding population (not a large release of many fish)
  • Stomach content analysis has documented lionfish predation of cleaner fish
  • Every site visited in the Berries in April contained lionfish – most contained multiple fish

Here is what we are working on with NOAA and Bahamian researchers:

  • Continuing documentation of lionfish distribution and impacts on local fish populations
  • Documentation of lionfish at cleaning stations and subsequent predation on cleaning fish
  • Predation by other species on lionfish
  • Genetic relationships of lionfish in one area (NC, northern Bahamas) to those in other areas (S Bahamas) to determine dispersion pathways.
  • Parasitology of lionfish (they appear to have few parasite compared to native fish)
  • Larval occurrence at different locations using larval light traps
  • Juvenile recruitment preference using small shallow water nets and trawls
  • Trap preference of adult lionfish
  • Lionfish recruitment rates to sites denuded of lionfish (i.e., recruitment pressure)
  • Recruitment of lionfish to artificial structures
  • And more!

As part of this effort, REEF has planned more research efforts through the end of 2007. Each project will include participation of scientists, researchers, and/or REEF staff. For a list of upcoming projects visit or e-mail

REEF Events 10/07

DEMA Raffle Prize. Print courtesy of Tom Isgar.
DEMA Raffle Prize. Print courtesy of Tom Isgar.

Here's what we're up to in the coming months:

October 31- November 3: DEMA Show in Orlando, FL. Come visit us at both 1133 and you could win a signed print by Tom Isgar by partaking in our DEMA raffle to help raise funds for REEF.

November 11-17: Conservation Week with Stuart Cove's Dive Bahamas in Nassau with Ned and Anna DeLoach, Bruce Purdy and Andy Dehart

Recent additions to the previously planned Eco-week at Stuart Coves Dive Bahamas in Nassau will be highlighted by Ned and Anna DeLoach, who will be presenting their famous behavior talks as part of the week's activities.  In addition, Andy Dehart, general Manager of the National Aquarium in Washington DC and Bruce Purdy, Bahamas dive operator and conservationist will talk about Bahamian conservation issues and marine protected areas. As previously planned, Lad Akins will lead the project and discuss lionfish issues as they relate to other environmental factors such as artificial reefs. Stuart Cove will host the project and discuss shark and local conservation issues.

December 8-14: Blackbeard's Cruises is announcing a new lionfish project focusing on Grand Bahama.

For more information, on these projects, view the pdf here...

Lloyd Bridges Scholarship Available for Akumal Field Survey

Beachfront at Bahia Principe in Akumal
Maya Glyph at Ruins of Tulum nearby to the Resort
Coral Reef Training for hotel staff at Bahia Principe
Lloyd Bridges as Mike Nelson, ex-Navy Frogman

Once again, CEDAM International is offering two scholarships for educators to participate on a REEF Field Survey. For the 2008 survey season, the scholarships will apply to the Gran Bahia Principe Field Survey in Akumal, Mexico. Dates for this trip are May 17-24, 2008. You can visit REEF's Field Survey page to view trip details and also check out the trip flyer. To apply for this scholarship, please visit the CEDAM website at then click on the Lloyd Bridges Scholarship tab at the top of the page to see details.

The two scholarships enable qualified educators to participate - at no cost - in a CEDAM-sponsored or -sanctioned expedition. This REEF Field Survey will be led by Joe Cavanaugh, REEF Field Operations Director. Participants will have the opportunity to collect data, participate in daily talks, and interact with REEF members, staff, and local organizations. This will be a hands-on experience during which participants have the opportunity for fish identification and marine conservation training in and out of the water. REEF has partnered with ReefAid and Reefcheck and Ecologica Bahia in 2007 to assist Gran Bahia Principe Resort with developing a monitoring and assessment protection plan for their nearshore reefs. Our cooperative efforts in conservation at Bahia Principe are making a difference in protecting their reefs and some of our class time will focus on these successes.

To be eligible, applicants must be a certified scuba diver, a teacher (elementary or secondary level), or actively engaged in an education program at an institution or environmental organization, such as an aquarium, science center, or relevant non-profit organization.The scholarship will be awarded on the basis of both merit and financial need. CEDAM International will cover the recipient’s airfare and REEF Field Survey expenses (excluding incidentals and personal expenses), on this educational adventure. Bahia Principe is an all-inclusive resort so acoomodation, diving, and meals are included. The application deadline will be earlier than last year's deadline since the Field Survey pariticipation dates are in late May this year. Please have your completed applications submitted by April 1, 2008.

Many of our members may be too young to have seen the television program, Sea Hunt, starring Lloyd Bridges that ran from 1958-1961. One important aspect of the series was that Bridges made a plea at the end of each episode to protect the oceans, an early ocean conservation pioneer. He was also involved in several organizations including the American Oceans Campaign and Heal the Bay, a Los Angeles-based conservation group. He inspired a generation of SCUBA divers and you may want to check out Wikipedia to read more about many of the people Bridges worked with on Sea Hunt. Incidentally, I discovered that Bridges learned to SCUBA dive once contracted for the show and that he was offered the role of Captain Kirk before William Shatner!

There are still spaces (4) available for the Akumal Field Survey, email to inquire.

All applicants must complete an application form and return it along with the required essay and two letters of recommendation, to CEDAM (by mail or electronically) by April 1, 2008. Good Luck!

Design by Joanne Kidd, development by Ben Weintraub