The Grouper Moon Project is the Caribbean’s oldest continuous grouper spawning aggregation research program, and represents one of the most advanced, multi-faceted tropical fisheries research programs in the world. Scientific products stemming from the project aim to support science-informed policies that will faciliatate healthy grouper fisheries in the Cayman Islands in the coming years, while maintaining the Cayman Islands’ global leadership in collaborative tropical fisheries research and management. Since 2001, REEF and our collaborators have published several scientific papers sharing results from the research.

    

Waterhouse, L, SA Heppell, CV Pattengill-Semmens, C McCoy, P Bush, BC Johnson, BX Semmens. 2020. Recovery of critically endangered Nassau grouper (Epinephelus striatus) in the Cayman Islands following targeted conservation actions. Proceedings of the National Academy of Sciences. Jan 2020, 201917132; DOI: 10.1073/pnas.1917132117.

This paper presents exciting results from the Grouper Moon Project, documenting conservation success for endangered Nassau Grouper. Results of the analysis showed that on both Little Cayman and Cayman Brac in the Cayman Islands, Nassau Grouper spawning aggregations have more than tripled in response to adaptive management by the Cayman Islands government over a decade. On Little Cayman, the aggregating population grew from around 1,200 fish in 2009 to over 7,000 in 2018. The study used a two-pronged approach that included tagging and video census data for monitoring and counting Nassau Grouper populations in an effort to more accurately estimate annual numbers of fish in the population, and thus provide insight into the effects of ongoing conservation efforts. While many governments have enacted regional or seasonal fishing closures in an attempt to allow recovery of overfished stocks of aggregating reef fishes, this is the first study to provide evidence that these measures can be successful across multiple decades. View PDF

Wilson, KC, BX Semmens, CV Pattengill-Semmens, C McCoy, A Širović. 2020. Potential for grouper acoustic competition and partitioning at a multispecies spawning site in Little Cayman, Cayman Islands. Marine Ecology Progress Series, 634: 127-146. doi:10.3354/meps13181.

This paper summarizes research done as part of the Grouper Moon Project to understand sound made by several species of grouper. Many fishes produce calls during spawning that aid in species and mate recognition. When multiple sound-producing species inhabit an area, the detection range may decrease and limit call function. Acoustic partitioning, the separation of calls in time, space, or spectral frequency, can minimize interference among species and provide information about fish behavior and ecology, including possible response to increasing anthropogenic noise. The authors investigated acoustic partitioning among 4 sound-producing epinephelids, Nassau Grouper Red Hind, Black Grouper, and Yellowfin Grouper, using passive acoustic data collected at the mass spawning aggregation site on the west end of Little Cayman in the Cayman Islands during the spawning seasons of 2015 to 2017. Results indicate separation in space and time between species calls, which aids in acoustic partitioning. When this separation did not occur, unique call structures were present, which may aid in effective intraspecies communication. View PDF

Wilson, KC, BX Semmens, SR Gittings, CV Pattengill-Semmens and A Širović. 2019. Development and evaluation of a passive acoustic localization method to monitor fish spawning aggregations and measure source levels. OCEANS 2019 MTS/IEEE SEATTLE, Seattle, WA, USA, 2019, pp. 1-7. doi: 10.23919/OCEANS40490.2019.8962663.

This work was presented at the OCEANS19 meeting, and was a result of research done as part of the Grouper Moon Project. More than 800 species of fish produce sound including Red Hind (Epinephelus guttatus), Nassau Grouper (E. striatus), Black Grouper (Mycteroperca bonaci), and Yellowfin Grouper (M. venenosa). Their sounds can be used to monitor these fish and may be a means to estimate abundance if parameters such as source levels, detection probabilities, and cue rates are known. During the week of Nassau Grouper spawning in February 2017, a passive acoustic array was deployed off Little Cayman in the Cayman Islands to study the temporal and spatial dynamics of spawning aggregations of Nassau Grouper and Red Hind, and measure the source levels of the sounds produced by all four species. The localization method was based on hyperbolic localization of cross-correlated time differences of arrival and its accuracy evaluated via simulations and empirical measurements. The high number of localizations at fish spawning aggregations suggests that localization can be used to accurately measure source levels, detection ranges, and other variables needed for density estimation of spawning aggregations. View paper online

Egerton, JP, AF Johnson, L Le Vay, CM McCoy, BX Semmens, SA Heppell, and JR Turner. 2017. Hydroacoustics for the discovery and quantification of Nassau grouper (Epinephelus striatus) spawning aggregations. Coral Reefs. 36 (2): 589-600

The Grouper Moon Project is always looking for new and/or better ways of accurately estimating the number of spawning Nassau Grouper at the aggregation sites being monitored. In 2014, we tested the use of a split-beam echosounder as a tool for surveying the abundance and size of fish at the aggregation site; the results of the study are detailed in this peer-reviewed paper. We found that the echosounder performs fairly well at providing an index of abundance, although the absolute accuracy of the method was not sufficient to replace other survey methods (e.g. mark and recapture monitoring). After calibrating the method with diver-based fish length surveys, the tool was able to accurately capture estimates of aggregating fish sizes. Surveys on all 3 islands (Little Cayman, Cayman Brac, and Grand Cayman) showed that the average size of Nassau Grouper on Little Cayman was significantly larger than on both Brac and Grand. On the other hand, the sizes of Nassau Grouper on Brac and Grand were not significantly different. Based on this study, the echosounder is a potentially useful tool for surveying aggregations, but is likely best use to complement more intensive diver-based survey methods. View paper online.

Bernard, AM, KA Feldheim, R Nemeth, E Kadison, J Blondeau, BX Semmens, MS Shivji. 2016. The ups and downs of coral reef fishes: the genetic characteristics of a formerly severely overfished but currently recovering Nassau grouper fish spawning aggregation. Coral Reefs. 35:273–284

Like many places throughout the Caribbean, Nassau Grouper spawning aggregations in the US Virgin Islands were overfished until their disappearance in the 1970s and 1980s. In the early 2000s, however, Nassau Grouper were found gathering at Grammanik Bank, USVI, a mesophotic coral reef adjacent to one of the extinct aggregation sites, and regulatory protective measures were implemented to protect this fledgling aggregation. The authors of this study addressed two objectives: 1) which factors (local vs. external recruitment) are important in shaping recovery of the USVI spawning aggregations, and 2) the impact of severe past overfishing on the genetic structure of the Gremmanik Bank aggregation. For this second objective, REEF Grouper Moon Project scientists provided genetic samples from individual Nassau Grouper taken from the Little Cayman spawning aggregation, a much larger and less impacted aggregation. No population structure was detected between the USVI and Cayman spawning aggregations. Additionally, the USVI spawning population showed signs of a genetic bottleneck, typical of greatly reduced populations. These collective results suggest that external recruitment is an important driver of the USVI spawning aggregation recovery. These findings also provide a baseline for future genetic monitoring of the spawning aggregations. View the paper online.

Archer, SK, JE Allgeier, BX Semmens, SA Heppell, CV Pattengill-Semmens, AD Rosemond, PG Bush, CM McCoy, BC Johnson, CA Layman. 2014. Hot Moments in Spawning Aggregations: implications for ecosystem-scale nutrient cycling. Coral Reefs. 10.1007/s00338-014-1208-4

This paper presents results from a study conducted as part of REEF's Grouper Moon Project, evaluating the potential ecosystem-level effect of Nassau Grouper aggregations. In particular, the study looked at the impact the spawning aggregation has in creating biogeochemical "hot moments", which occur when a temporary increase in availability of one or more limiting nutrients results in elevated rates of biogeochemical reactions. In this case, the limited nutrients are nitrogen and phosphorus, and the temporary increase is resulting from all of the grouper poop that results when approximately 5,000 Nassau Grouper gather in a small area for 10 days during the spawning season. View the paper online.

Jackson, AM, BX Semmens, Y Sadovy de Mitcheson, RS Nemeth, SA Heppell, PG Bush, A Aguilar-Perera, JAB Claydon, MC Calosso, KS Sealey, MT Schärer, G Bernardi. 2014. Population structure and phylogeography in Nassau Grouper (Epinephelus striatus), a mass-aggregating marine fish. PLoS ONE. DOI: 10.1371/journal.pone.0097508

This study, co-authored by scientists from REEF's Grouper Moon Project, evaluated genetic connectedness between Nassau Grouper populations throughout the Caribbean. The authors obtained genetic tissue samples from 620 Nassau Grouper from 19 sites across 9 countries, including the Cayman Islands. They found evidence for strong genetic differentiation among Nassau Grouper subpopulations throughout the Caribbean. These results suggest that, despite a lack of physical barriers, Nassau grouper form multiple distinct subpopulations in the Caribbean Sea. Oceanography (regional currents, eddies) likely plays an important role in retaining larvae close to spawning sites at both local and regional spatial scales. These findings highlight the importance of conservation initiatives such at REEF's Grouper Moon program in the Cayman Islands. View the paper online.

Jackson, AM, BX Semmens, and G Bernardi. 2012. Characterization and cross-species amplification of microsatellite markers in Nassau grouper (Epinephelus striatus). Molecular Ecology Resources. 12(5): 972- 974.

This paper is part of the larger body of genetic research being conducted on Nassau Grouper in the Caribbean. View PDF

Heppell, SA, BX Semmens, SK Archer, CV Pattengill-Semmens, PG Bush, CM McCoy, SS Heppell, BC Johnson. 2012. Documenting recovery of a spawning aggregation through size frequency analysis from underwater laser calipers measurements. Biological Conservation. 155: 119-127.

This paper presents a key technique that scientists from REEF and our Grouper Moon collaborators have used to monitor fish on the Little Cayman spawning aggregation that does not require the capture and handling of fish. We show that length-distribution data can be collected by divers using a video-based system with parallel lasers calibrated to a specific distance apart, and subsequently use those data to monitor changes in the size distribution over time. View PDF

Archer, S.K., Scott A Heppell, Brice X Semmens,Christy V Pattengill-Semmens, Phillippe G Bush, Croy M McCoy, Bradley C Johnson. 2012. Patterns of color phase indicate spawn timing at a Nassau grouper Epinephelus striatus spawning aggregation. Current Zoology. 58(1): 73 - 83

This paper summarizes shifts if color phase of individual Nassau grouper at the Little Cayman spawning site in the days leading up to spawning, based on 5 years of video data. View PDF 

Semmens BX, PG Bush, S Heppell, B Johnson, C McCoy, CV Pattengil-Semmens, and L Whaylen. 2007. Charting a Course for Nassau Grouper Recovery in the Caribbean: What We’ve Learned and What We Still Need to Know. 60th Gulf and Caribbean Fisheries Institute Meeting Proceedings.

Nassau Grouper (Epinephelus striatus) were historically one of the most important shallow water fisheries in the Caribbean, yet now are rarely taken. Although normally solitary, during the winter full moon Nassau grouper attend aggregations at spawning site to reproduce. Now, however, there are only a handful of known Nassau Grouper aggregations with more than 1,000 fish left in the Caribbean. Why has the species declined so precipitously, and what can be done to reverse the trend? These topics were explored during a presentation given by Grouper Moon researchers at the 2007 Gulf and Caribbean Fisheries Institute conference. The researchers presented recent and on-going research into the behavior and ecology of Nassau Grouper as part of the Grouper Moon Project, and highlight the conservation implications of this work. They also discussed critical research needs in the ongoing effort to identify prudent conservation measures for this species, including: habitat capacity, depensation, and recruitment variability. View PDF

Whaylen, L., P.G. Bush, B.C. Johnson, K.E. Luke, C.M.R. McCoy, S. Heppell, B. X. Semmens, and M. Boardman. 2006. Aggregation dynamics and lessons learned from five years of monitoring at a Nassau grouper (Epinephelus striatus) spawning aggregation in Little Cayman, Cayman Islands, BWI. 59th Gulf and Caribbean Fisheries Institute Meeting Proceedings. 59:1-14

This paper summarized the first five years of monitoring of the Little Cayman West End aggregation, including a summary of spawning activity, total numbers of fish present at the aggregation each year, coloration, and behavior. View PDF

Semmens, B.X., K.E. Luke, P.G. Bush, C.M.R. McCoy, and B.C. Johnson. 2006. Isopod infestation of post-spawning Nassau grouper around Little Cayman Island. Journal of Fish Biology 69: 933-937

This paper documents attacks by the isopod Excorallana tricornis tricornis on Nassau grouper caught in Antillian fish traps during the post-spawning season of Spring 2005. Fish were being trapped in order to acoustically tag individuals from sites around Little Cayman Island in order to better understand what percentage of reproductive-size individuals attend the aggregation each year. The paper discusses the apparent energetic costs associated with spawning. This work was also presented as a posted at the 56th Gulf and Caribbean Fisheries Institute Meeting in 2005 (View PDF), and a summary of the poster is posted online. (View Poster)

Taylor, JC, DB Eggleston, and PS Rand. 2006. Nassau grouper (Epinephelus striatus) spawning aggregations: hydroacoustic surveys and geostatistical analysis. NOAA Professional Paper. NMFS 5: 18-25.

In 2006, researchers from Univeristy of North Carolina Willmington collaborated with Grouper Moon Project researchers from REEF and Cayman Island Department of Environment to conduct mobile hydroacoustic surveys on the Little Cayman spawning aggregation site. Hydroacoustics, which is similar to a fish finder device found on a recreational fishing boat, but with better resolution and technology, has emerged as a valuable tool in fishery population assessments. The goal of the study was to determine the utility of this emerging technology to assist in the estimation of density, spatial extent, and total abundance of a Nassau grouper. This report summarizes the field effort and findings. View PDF

Semmens, B.X., K.E. Luke, P.G. Bush, C.V. Pattengill-Semmens, B. Johnson, C. McCoy and S. Heppell. 2005. Investigating the reproductive migration and spatial ecology of Nassau grouper (Epinephelus striatus) on Little Cayman Island using acoustic tags – An Overview. 56th Gulf and Caribbean Fisheries Institute Meeting Proceedings. 56:1-8

This paper provides an overview of the acoustic tagging project that was initiated on Little Cayman Island in 2005. The project aims to better understand the sphere of influence that a spawning aggregation has on the island's population of Nassau grouper, as well the impact of harvest protections on local fish densities. View PDF

Whaylen, L., Pattengill-Semmens, C.V., Semmens, B.X., Bush, P.G. and M.R. Boardman. 2004. Observations of a Nassau Grouper (Epinephelus striatus) Spawning Aggregation Site In Little Cayman, Including Multi-Species Spawning Information. Environmental Biology of Fishes. 70: 305-313

This paper summarizes the findings from the 2002 REEF Grouper Moon Project, which documented the characteristics of a newly discovered Nassau grouper spawning aggregation. View PDF