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This study analyzed temporal trends of the yellow stingray in the Florida Keys National Marine Sanctuary (FKNMS) as recorded by trained volunteer divers using the Roving Diver Technique. Data were obtained from the REEF Fish Survey Project. A generalized linear model on presence-absence data was used to estimate the change in yellow stingray by year. Habitat type, bottom time, depth, site, water temperature, and Julian date were included in the model to standardize the data. The decline in sighting frequency has occurred in all habitat types, depths, sites, and regions of the FKNMS.